In unlicensed spectrum, wireless communications employing carrier sense multiple access with collision avoidance (CSMA/CA) suffer from longer transmission delay time as the number of user terminals (UTs) increases, because packet collisions are more likely to occur. To cope with this problem, this paper proposes a new multiuser detection (MUD) scheme that uses both request-to-send (RTS) and enhanced clear-to-send (eCTS) for high-reliable and low-latency wireless communications. As in conventional MUD scheme, the metric-combining MUD (MC-MUD) calculates log likelihood functions called metrics and accumulates the metrics for the maximum likelihood detection (MLD). To avoid increasing the number of states for MLD, MC-MUD forces the relevant UTs to retransmit their packets until all the collided packets are correctly detected, which requires a kind of central control and reduces the system throughput. To overcome these drawbacks, the proposed scheme, which is referred to as cancelling MC-MUD (CMC-MUD), deletes replicas of some of the collided packets from the received signals, once the packets are correctly detected during the retransmission. This cancellation enables new UTs to transmit their packets and then performs MLD without increasing the number of states, which improves the system throughput without increasing the complexity. In addition, the proposed scheme adopts RTS and eCTS. One UT that suffers from packet collision transmits RTS before the retransmission. Then, the corresponding access point (AP) transmits eCTS including addresses of the other UTs, which have experienced the same packet collision. To reproduce the same packet collision, these other UTs transmit their packets once they receive the eCTS. Computer simulations under one AP conditions evaluate an average carrier-to-interference ratio (CIR) range in which the proposed scheme is effective, and clarify that the transmission delay time of the proposed scheme is shorter than that of the conventional schemes. In two APs environments that can cause the hidden terminal problem, it is demonstrated that the proposed scheme achieves shorter transmission delay times than the conventional scheme with RTS and conventional CTS.