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. 2004 Jun 15;64(12):4286-93.
doi: 10.1158/0008-5472.CAN-03-3084.

Pivotal role of the cell death factor BNIP3 in ceramide-induced autophagic cell death in malignant glioma cells

Shigeru Daido  1 , Takao Kanzawa, Akitsugu Yamamoto, Hayato Takeuchi, Yasuko Kondo, Seiji Kondo
Affiliations

Pivotal role of the cell death factor BNIP3 in ceramide-induced autophagic cell death in malignant glioma cells

Shigeru Daido et al. Cancer Res. .

Abstract

The sphingolipid ceramide has been recognized as an important second messenger implicated in regulating diverse signaling pathways especially for apoptosis. Very little is known, however, about the molecular mechanisms underlying nonapoptotic cell death induced by ceramide. In the present study, we first demonstrate that ceramide induces nonapoptotic cell death in malignant glioma cells. The cell death was accompanied by several specific features characteristic of autophagy: presence of numerous autophagic vacuoles in the cytoplasm, development of the acidic vesicular organelles, autophagosome membrane association of microtubule-associated protein light chain 3 (LC3), and a marked increase in expression levels of two forms of LC3 protein (LC3-I and LC3-II). We additionally demonstrate that ceramide decreases mitochondrial membrane potential and activates the transcription of death-inducing mitochondrial protein, BNIP3, resulting in increased expression levels of its mRNA and protein in malignant glioma cells. Moreover, tumor cells transfected with BNIP3 gene undergo autophagy in the absence of ceramide. These results suggest that ceramide induces autophagic cell death in malignant glioma cells via activation of BNIP3. This study adds a new concept to characterize the pathways by which ceramide acts to induce nonapoptotic autophagic cell death in malignant gliomas.

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