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Clinical Significance of Human Kallikrein7 Gene Expression in Colorectal Cancer

  • Translational Research and Biomarkers
  • Published:
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Abstract

Background

The human kallikrein-related peptidases (KLK) are considered important prognostic biomarkers in cancer. The aim of the current study is to demonstrate gene expression of KLK7 in colorectal cancer (CRC) and to correlate the relative KLK7 expression level with clinicopathological factors of CRC.

Methods

KLK7 messenger RNA (mRNA) expression was examined in nine CRC cancer cell lines by real-time polymerase chain reaction. The expression levels of KLK7 mRNA in cancerous tissues (n = 136) and paired normal tissues (n = 136) of CRC patients were also examined.

Results

Six of the nine cell lines expressed the KLK7 gene. KLK7 mRNA expression levels in cancer tissues were significantly higher than those in normal tissues. Multivariate analysis revealed that the KLK7 mRNA expression level in cancer was an independent prognostic factor, especially in liver metastasis.

Conclusions

We provide evidence suggesting that KLK7 mRNA expression is correlated with prognosis in CRC patients, especially in liver metastasis.

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Acknowledgment

The funding sources supporting our work are as follows: Grant sponsor: Japan Society for the Promotion of Science (JSPS). Grant-in-Aid for Scientific Research; Grant numbers: 17109013,18659384, 18390367, 18590333, 19591509, 19390336, 20390360, 20591547, 20790961, 20659209, 20790960; Grant sponsor: The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas; Grant number: 18015039; Grant sponsor: Third-Term Comprehensive 10-year Strategy for Cancer Control; Grant number: 16271201; Grant sponsors: CREST, Japan Science and Technology Agency (JST); NEDO (New Energy and Industrial Technology Development Organization) Technological Development for Chromosome Analysis; The Ministry of Education, Culture, Sports, Science, and Technology of Japan for Scientific Research on Priority Areas, Cancer Translational Research Project, Japan; Grant of Clinical Research Foundation (2008–2010).

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Authors and Affiliations

  1. Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie, Japan

    Yasuhiro Inoue MD, Takeshi Yokoe MD, Yuji Toiyama MD, Chikao Miki MD & Masato Kusunoki MD

  2. Department of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Kyushu, Japan

    Takehiko Yokobori MD & Koshi Mimori MD

  3. Department of Surgery, Osaka University Graduate School of Medicine, Osaka, Japan

    Masaki Mori MD

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  1. Yasuhiro Inoue MD
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  2. Takehiko Yokobori MD
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Correspondence to Yasuhiro Inoue MD.

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Inoue, Y., Yokobori, T., Yokoe, T. et al. Clinical Significance of Human Kallikrein7 Gene Expression in Colorectal Cancer. Ann Surg Oncol 17, 3037–3042 (2010). https://doi.org/10.1245/s10434-010-1132-y

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  • DOI: https://doi.org/10.1245/s10434-010-1132-y

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