Understanding the Origins of Bacterial Resistance to Aminoglycosides through Molecular Dynamics Mutational Study of the Ribosomal A-Site
Figure 1
The binding site of paromomycin.
(a) The sequence of the paromomycin binding site in the 16S ribosomal RNA (E. coli numbering); the mutated bases in our MD study are colored light gray and the arrows depict the applied mutations; the A1408G substitution accounts for the most important difference between the prokaryotic (A) and eukaryotic (G) sequence of the A-site and was previously analyzed [33]. The complete simulated structure contains two symmetric binding sites (A-sites) as in the crystal structure (PDB entry 1J7T). (b) A stick model of paromomycin heavy atoms showing atom names and ring numbering.