Ecofriendly Method for Bioremediation of Chlorpyrifos from Agricultural Soil by Novel Fungus Aspergillus terreus JAS1 | Water, Air, & Soil Pollution Skip to main content

Advertisement

Springer Nature Link
Log in

Ecofriendly Method for Bioremediation of Chlorpyrifos from Agricultural Soil by Novel Fungus Aspergillus terreus JAS1

  • Published:
Water, Air, & Soil Pollution Aims and scope Submit manuscript

Abstract

Biodegradation of chlorpyrifos was studied in mineral medium and soil with a novel fungal strain JAS1 isolated from a paddy field soil. The molecular characterization based on 18S rRNA sequence homology confirmed its identity as Aspergillus terreus. The 300-mg L−1 chlorpyrifos and its major metabolite 3,5,6-trichloro-2-pyridinol (TCP) were completely degraded within 24 h of incubation in the mineral medium. In soil enriched with chlorpyrifos and nutrients (carbon, nitrogen, and phosphorous), A. terreus JAS1 was able to degrade chlorpyrifos and its metabolite TCP (300 mg kg−1 soil) in 24 and 48 h, respectively. The soil was spiked with chlorpyrifos (300 mg kg−1 soil) devoid of nutrients and the fungal strain was capable of degrading both chlorpyrifos and TCP in 24 and 48 h, respectively. The course of the degradation process was studied using high-performance liquid chromatography and Fourier transform infrared analyses. These results showed that the chlorpyrifos-degrading fungal strain had the potential to degrade the pesticide-contaminated agricultural soils even without addition of nutrients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
¥17,985 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Anwar, S., Liaquat, F., Khan, Q. M., Khalid, Z. M., & Iqbal, S. (2009). Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by Bacillus pumilus strain C2A1. Journal of Hazardous Materials, 168, 400–405.

    Article  CAS  Google Scholar 

  • Bailey, H. C., DiGiorgio, C., Kroll, K., Miller, J. L., Hinton, D. E., & Starrett, G. (1996). Development of procedures for identifying pesticide toxicity in ambient waters: Carbofuran, diazinon, chlorpyrifos. Environmental Toxicology and Chemistry, 15, 837–845.

    Article  CAS  Google Scholar 

  • Bending, G. D., Friloux, M., & Walker, A. (2002). Degradation of contrasting pesticides by white rot fungi and its relationship with ligninolytic potential. FEMS Microbiology Letter, 212, 59–63.

    Article  CAS  Google Scholar 

  • Bhalerao, T. S., & Puranik, P. R. (2007). Biodegradation of organochlorine pesticide, endosulfan, by a fungal soil isolate, Aspergillus niger. International Biodeterioration and Biodegradation, 59, 315–321.

    Article  CAS  Google Scholar 

  • Bhalerao, T. S., & Puranik, P. R. (2009). Microbial degradation of monocrotophos by Aspergillus oryzae. International Biodeterioration and Biodegradation, 63, 503–508.

    Article  CAS  Google Scholar 

  • Bumpus, J. A., Kakar, S. N., & Coleman, R. D. (1993). Fungal degradation of organophosphorous insecticides. Applied Biochemistry and Biotechnology, 39(40), 715–726.

    Article  Google Scholar 

  • Fang, H., Xiang, Y. Q., Hao, Y. J., Chu, X. Q., Pan, X. D., Yu, J. Q., & Yu, Y. L. (2008). Fungal degradation of chlorpyrifos by Verticillium sp. DSP in pure cultures and its use in bioremediation of contaminated soil and pakchoi. International Biodeterioration and Biodegradation, 61, 294–303.

    Article  CAS  Google Scholar 

  • Greenhalgh, R., Dhavan, K. L., & Weinberger, P. (1980). Hydrolysis of fenitrothion in model and natural aquatic systems. Journal of Agricultural and Food Chemistry, 28, 102–105.

    Article  CAS  Google Scholar 

  • Horne, I., Sutherland, T. D., Oakeshott, J. G., & Russell, R. J. (2002). Cloning and expression of the phosphotriesterase gene hocA from Pseudomonas monteilii C11. Microbiology, 148, 2687–2695.

    CAS  Google Scholar 

  • Karpouzas, D. G., & Walker, A. (2000). Factors influencing the ability of Pseudomonas putida epI to degrade ethoprophos in soil. Soil Biology and Biochemistry, 32, 1753–1762.

    Article  CAS  Google Scholar 

  • Li, X., Jiang, J., Gu, L., Ali, S. W., He, J., & Li, S. (2008). Diversity of chlorpyrifos-degrading bacteria isolated from chlorpyrifos-contaminated samples. International Biodeterioration and Biodegradation, 62, 331–335.

    Article  CAS  Google Scholar 

  • Mallick, B. K., Banerji, A., Shakil, N. A., & Sethunathan, N. N. (1999). Bacterial degradation of chlorpyrifos in pure culture and in soil. Bulletin of Environment Contamination and Toxicology, 62, 48–55.

    Article  CAS  Google Scholar 

  • Martin, A. R., Calva, G. C., Avelizapa, N. R., Diaz-Cervantes, M. D., & Vazquez, R. R. (2007). Solid culture amended with small amounts of raw coffee beans for the removal of petroleum hydrocarbon from weathered contamination soil. International Biodeterioration and Biodegradation, 60, 35–39.

    Article  CAS  Google Scholar 

  • Pino, N., & Penuela, G. (2011). Simultaneous degradation of the pesticides methyl parathion and chlorpyrifos by an isolated bacterial consortium from a contaminated site. International Biodeterioration and Biodegradation, 65, 827–831.

    Article  CAS  Google Scholar 

  • Racke, K. D., Coats, J. R., & Titus, K. R. (1988). Degradation of chlorpyrifos and its hydrolysis products, 3,5,6-trichloro-2-pyridinol, in soil. Jouranl of Environmental Science and Health B, 23, 527–539.

    Article  Google Scholar 

  • Racke, K. D., Steele, K. P., Yoder, R. N., Dick, W. A., & Avidov, E. (1996). Factors effecting the hydrolytic degradation of chlorpyrifos in soil. Journal of Agricultural and Food Chemistry, 44, 1582–1592.

    Article  CAS  Google Scholar 

  • Shukla, G., Kumar, A., Bhanti, M., Joseph, P. E., & Taneja, A. (2006). Organochlorine pesticide contamination of ground water in the city of Hyderabad. Environment International, 32, 244–247.

    Article  CAS  Google Scholar 

  • Siddique, T., Okeke, B. C., Arshad, M., & Frankenberger, W. T. (2003). Biodegradation kinetics of endosulfan by Fusarium ventricosum and a Pandoraea species. Journal of Agricultural and Food Chemistry, 51, 8015–8019.

    Article  CAS  Google Scholar 

  • Singh, B. K., Kuhad, R. C., Singh, A., & Lal, R. (1999). Biochemical and molecular basis of pesticide degradation by microorganisms. Critical Reviews in Biotechnology, 19, 197–225.

    Article  CAS  Google Scholar 

  • Singh, B. K., Walker, A., Morgan, J. A., & Wright, D. J. (2003). Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Applied and Environmental Microbiology, 69, 5198–5206.

    Article  CAS  Google Scholar 

  • Yang, L., Zhao, Y., Zhang, B., Yang, C. H., & Zhang, X. (2005). Isolation and characterization of a chlorpyrifos and TCP degrading bacterium. FEMS Microbiology Letter, 251, 67–73.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors greatly acknowledge the Department of Science and Technology (DST), New Delhi for providing a grant (no. DST/TSG/NTS/2009/67) for this research work.

Author information

Authors and Affiliations

  1. Microbial Biotechnology Laboratory, School of Biosciences and Technology, Division of Environmental Biotechnology, VIT University, Vellore, 632014, Tamil Nadu, India

    Sivagnanam Silambarasan & Jayanthi Abraham

Authors
  1. Sivagnanam Silambarasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jayanthi Abraham
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jayanthi Abraham.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Silambarasan, S., Abraham, J. Ecofriendly Method for Bioremediation of Chlorpyrifos from Agricultural Soil by Novel Fungus Aspergillus terreus JAS1. Water Air Soil Pollut 224, 1369 (2013). https://doi.org/10.1007/s11270-012-1369-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11270-012-1369-0

Keywords

Search

Navigation