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An electrochemical chlorpyrifos aptasensor based on the use of a glassy carbon electrode modified with an electropolymerized aptamer-imprinted polymer and gold nanorods

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Abstract

A highly selective and sensitive aptasensor is described for voltammetric determination of the pesticide chlorpyrifos (CPS). The sensor was constructed by modifying a glassy carbon electrode (GCE) with gold nanorods and a polymer that was molecularly imprinted with an aptamer against CPS. This results in double specific recognition. Under optimal conditions and a working potential as low as 0.22 V (vs. Ag/AgCl), the nanotools has a dynamic range that covers the 1.0 fM - 0.4 pM CPS concentration range, and the detection limit is 0.35 fM. This is lower than any of the previously reported methods. This MIP-aptasensor is selective over structural analogs, stable, and adequately reproducible. It was successfully applied to the determination of CPS in spiked food samples.

Impedimetric detection of Chlorpyrifos by using a Fe(CN)63−/4- probe based on double recognition of aptamer-molecular imprinted polymer onto a glassy carbon electrode modified with gold nanorod nanocomposite. The incubation with Chlorpyrifos lead to an increase of electron transfer resistance.

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Authors and Affiliations

  1. Department of Chemistry, Ilam University, PO. Box 69315-516, Ilam, Iran

    Mahmoud Roushani

  2. Department of Chemistry, Payame Noor University, PO. Box19395-4697, Tehran, Iran

    Azizollah Nezhadali & Zeynab Jalilian

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  1. Mahmoud Roushani
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  2. Azizollah Nezhadali
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Correspondence to Mahmoud Roushani.

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Roushani, M., Nezhadali, A. & Jalilian, Z. An electrochemical chlorpyrifos aptasensor based on the use of a glassy carbon electrode modified with an electropolymerized aptamer-imprinted polymer and gold nanorods. Microchim Acta 185, 551 (2018). https://doi.org/10.1007/s00604-018-3083-0

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