Zusammenfassung
Ein neuartiges miniaturisiertes Analysesystem zur quantitativen Zellanalyse, auf Basis dielektrischer Mikrosensoren und Mikrofluidik, wird in dieser Arbeit vorgestellt. Das realisierte Lab-on-a-Chip beinhaltet in Kammern eingebettete, passivierte interdigitale Elektrodensysteme. Die Einführung einer Multilagen-Passivierung ermöglicht, im Gegensatz zu herkömmlichen Bioimpedanz-Systemen, die Isolation und somit die räumliche Trennung der dielektrischen Mikrosensoren von der Flüssigkeitsumgebung in den Analysekammern. Anhand von unterschiedlichen, in vitro cultivated cells. The overall performance of the system is demonstrated on various bacterial and yeast strains. Due to the high sensitivity of the contact-less dielectric microsensors it is possible to directly identify microbial strains, based on morphological differences and biological composition in the absence of any indicators or labels. Additionally, dielectric changes occurring in sub-cellular structures such as membranes can be directly monitored over a wide frequency range. As a result, microfluidic biochips are developed to continuously monitor cell morphology changes in a non-invasive manner over long periods of time.
Summary
In the presented work we describe the novel combination of contact-less dielectric microsensors and microfluidics for quantitative cell analysis. The lab-on-a-chip system consists of microfluidic channels and chambers together with integrated and passivated interdigitated electrode structures. In contrast to existing bioimpedance methods implemented for cell analysis, the dielectric microsensors are completely insulated and physically removed from the liquid sensing environment using defined multi-passivation layer of distinct size and composition. Consequently, these structures act as contact-less microsensors for the characterization of in vitro kultivierten, Bakterien und Hefezellen wird das Lab-on-a-Chip charakterisiert. Es zeigt sich, dass mikrobiologische Substanzen aufgrund morphologischer Unterschiede bzw. ihrer biologischen Zusammensetzung ohne Verwendung von Markern oder Indikatoren identifiziert werden können. Dielektrische Variationen in subzellularen Strukturen, wie beispielsweise der Membranen, sind über einen weiten Messfrequenzbereich beobachtbar. Der präsentierte mikrofluidische Biochip wurde speziell für die kontinuierliche und nicht-invasive Beobachtung der Zellmorphologie über lange Zeiträume entwickelt.
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Ertl, P., Heer, R. Interdigitated impedance sensors for analysis of biological cells in microfluidic biochips. Elektrotech. Inftech. 126, 47–50 (2009). https://doi.org/10.1007/s00502-009-0607-7
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DOI: https://doi.org/10.1007/s00502-009-0607-7