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Generation and Interpretation of Context-Specific Human Protein–Protein Interaction Networks with HIPPIE

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Protein-Protein Interaction Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2074))

Abstract

High-throughput techniques for the detection of protein–protein interactions (PPIs) have enabled a systems approach for the study of the living cell. However, the increasing amount of protein interaction data, the varying quality of these measurements, and the lack of context information make it difficult to construct meaningful and reliable protein networks.

The Human Integrated Protein–Protein Interaction rEference (HIPPIE) is a web tool that integrates and annotates experimentally supported human PPIs from a heterogeneous set of data sources. In HIPPIE, one can query for the interactors of one or more proteins and generate high-quality and context-specific networks. This chapter highlights HIPPIE’s most important features and exemplifies its functionality through a proposed use case.

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Acknowledgments

HIPPIE has been developed and is maintained in the lab of Prof. Miguel Andrade. He also gave valuable feedback on this book chapter. We also thank the Zentrum für Datenverarbeitung of the Johannes Gutenberg Universität for their help in the maintenance of the web server that hosts HIPPIE.

Author information

Authors and Affiliations

  1. Human Embryo and Stem Cell Laboratory, The Francis Crick Institute, London, UK

    Gregorio Alanis-Lobato

  2. Department of Experimental Oncology, European Institute of Oncology, Milan, Italy

    Martin H. Schaefer

Authors
  1. Gregorio Alanis-Lobato
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  2. Martin H. Schaefer
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Corresponding authors

Correspondence to Gregorio Alanis-Lobato or Martin H. Schaefer .

Editor information

Editors and Affiliations

  1. Gene Center, Ludwig-Maximilians-Universität München, München, Germany

    Stefan Canzar

  2. Gene Center, Ludwig-Maximilians-Universität München, München, Germany

    Francisca Rojas Ringeling

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Alanis-Lobato, G., Schaefer, M.H. (2020). Generation and Interpretation of Context-Specific Human Protein–Protein Interaction Networks with HIPPIE. In: Canzar, S., Ringeling, F. (eds) Protein-Protein Interaction Networks. Methods in Molecular Biology, vol 2074. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9873-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9873-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9872-2

  • Online ISBN: 978-1-4939-9873-9

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