查看nginx 错误日志：

GetFileAttributesEx() "D:\linuxdir\php  est" failed (123: The filename, directory name, or volume label syntax is incorrect), client: 127.0.0.1, server: test.test, request: "GET / HTTP/1.1", host: "test.test"
2019/03/04 15:59:15 [crit] 3860#3096: *22 CreateFile() "D:\linuxdir\php est/favicon.ico" failed (123: The filename, directory name, or volume label syntax is incorrect), client: 127.0.0.1, server: test.test, request: "GET /favicon.ico HTTP/1.1", host: "test.test", referrer: "http://test.test/"

解决方案:

打开：vhosts.conf

phpstudy 用nginx，报500，错误日志：CreateFile_HTTP

在项目目录前面 添加 \解决

再次访问，500错误不存在了，可以正常访问了，所以，在ngnix配置文件里要注意不要有转义符。