{"id":"https://openalex.org/W4225159435","doi":"https://doi.org/10.1117/1.jei.31.2.023041","title":"Automatic thresholding method for high-throughput digital polymerase chain reaction fluorescence images with uneven illumination","display_name":"Automatic thresholding method for high-throughput digital polymerase chain reaction fluorescence images with uneven illumination","publication_year":2022,"publication_date":"2022-04-29","ids":{"openalex":"https://openalex.org/W4225159435","doi":"https://doi.org/10.1117/1.jei.31.2.023041"},"language":"en","primary_location":{"is_oa":false,"landing_page_url":"https://doi.org/10.1117/1.jei.31.2.023041","pdf_url":null,"source":{"id":"https://openalex.org/S158511090","display_name":"Journal of Electronic Imaging","issn_l":"1017-9909","issn":["1017-9909","1560-229X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false},"type":"article","type_crossref":"journal-article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100397991","display_name":"Yue Yu","orcid":"https://orcid.org/0000-0002-9865-2212"},"institutions":[{"id":"https://openalex.org/I75867142","display_name":"Xiamen University of Technology","ror":"https://ror.org/01285e189","country_code":"CN","type":"education","lineage":["https://openalex.org/I75867142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yue Yu","raw_affiliation_strings":["Xiamen University of Technology, Department of Communication Engineering, Xiamen"],"affiliations":[{"raw_affiliation_string":"Xiamen University of Technology, Department of Communication Engineering, Xiamen","institution_ids":["https://openalex.org/I75867142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109295055","display_name":"Qin Zhu","orcid":null},"institutions":[{"id":"https://openalex.org/I75867142","display_name":"Xiamen University of Technology","ror":"https://ror.org/01285e189","country_code":"CN","type":"education","lineage":["https://openalex.org/I75867142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qin Zhu","raw_affiliation_strings":["Xiamen University of Technology, Department of Communication Engineering, Xiamen"],"affiliations":[{"raw_affiliation_string":"Xiamen University of Technology, Department of Communication Engineering, Xiamen","institution_ids":["https://openalex.org/I75867142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069716714","display_name":"Lin Chen","orcid":"https://orcid.org/0000-0001-7943-3172"},"institutions":[{"id":"https://openalex.org/I75867142","display_name":"Xiamen University of Technology","ror":"https://ror.org/01285e189","country_code":"CN","type":"education","lineage":["https://openalex.org/I75867142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lin Chen","raw_affiliation_strings":["Xiamen University of Technology, Department of Communication Engineering, Xiamen"],"affiliations":[{"raw_affiliation_string":"Xiamen University of Technology, Department of Communication Engineering, Xiamen","institution_ids":["https://openalex.org/I75867142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100650080","display_name":"Xuyang Zhang","orcid":"https://orcid.org/0000-0003-2165-2588"},"institutions":[{"id":"https://openalex.org/I75867142","display_name":"Xiamen University of Technology","ror":"https://ror.org/01285e189","country_code":"CN","type":"education","lineage":["https://openalex.org/I75867142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xuyang Zhang","raw_affiliation_strings":["Xiamen University of Technology, Department of Communication Engineering, Xiamen"],"affiliations":[{"raw_affiliation_string":"Xiamen University of Technology, Department of Communication Engineering, Xiamen","institution_ids":["https://openalex.org/I75867142"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5074471361","display_name":"Jun Tang","orcid":"https://orcid.org/0009-0006-4924-4921"},"institutions":[{"id":"https://openalex.org/I75867142","display_name":"Xiamen University of Technology","ror":"https://ror.org/01285e189","country_code":"CN","type":"education","lineage":["https://openalex.org/I75867142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jun Tang","raw_affiliation_strings":["Xiamen University of Technology, Department of Communication Engineering, Xiamen"],"affiliations":[{"raw_affiliation_string":"Xiamen University of Technology, Department of Communication Engineering, Xiamen","institution_ids":["https://openalex.org/I75867142"]}]}],"institution_assertions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.156,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.376398,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":60,"max":70},"biblio":{"volume":"31","issue":"02","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"primary_topic":{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9976,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9976,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9939,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10981","display_name":"Cytomegalovirus and herpesvirus research","score":0.9921,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/otsus-method","display_name":"Otsu's method","score":0.52560425}],"concepts":[{"id":"https://openalex.org/C191178318","wikidata":"https://www.wikidata.org/wiki/Q2256906","display_name":"Thresholding","level":3,"score":0.7303231},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.7245565},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.7133879},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.689211},{"id":"https://openalex.org/C89600930","wikidata":"https://www.wikidata.org/wiki/Q1423946","display_name":"Segmentation","level":2,"score":0.6590956},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.6208881},{"id":"https://openalex.org/C21729346","wikidata":"https://www.wikidata.org/wiki/Q2444417","display_name":"Otsu's method","level":4,"score":0.52560425},{"id":"https://openalex.org/C32653426","wikidata":"https://www.wikidata.org/wiki/Q3813641","display_name":"Background subtraction","level":3,"score":0.5089271},{"id":"https://openalex.org/C124504099","wikidata":"https://www.wikidata.org/wiki/Q56933","display_name":"Image segmentation","level":3,"score":0.44864613},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.42301154},{"id":"https://openalex.org/C42781572","wikidata":"https://www.wikidata.org/wiki/Q1250322","display_name":"Digital image","level":4,"score":0.42239505},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.4219477},{"id":"https://openalex.org/C9417928","wikidata":"https://www.wikidata.org/wiki/Q1070689","display_name":"Image processing","level":3,"score":0.35745558},{"id":"https://openalex.org/C160633673","wikidata":"https://www.wikidata.org/wiki/Q355198","display_name":"Pixel","level":2,"score":0.22259945},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.19083756},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.1830833},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.12719509},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"is_oa":false,"landing_page_url":"https://doi.org/10.1117/1.jei.31.2.023041","pdf_url":null,"source":{"id":"https://openalex.org/S158511090","display_name":"Journal of Electronic Imaging","issn_l":"1017-9909","issn":["1017-9909","1560-229X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false}],"best_oa_location":null,"sustainable_development_goals":[],"grants":[{"funder":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China","award_id":"61701422"}],"datasets":[],"versions":[],"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3214851576","https://openalex.org/W3176725037","https://openalex.org/W308191704","https://openalex.org/W2769778947","https://openalex.org/W2741233822","https://openalex.org/W2536634271","https://openalex.org/W2350588503","https://openalex.org/W2146598241","https://openalex.org/W2110230079","https://openalex.org/W2069632396"],"abstract_inverted_index":{"The":[0,155],"demand":[1],"for":[2,100,178],"nucleic":[3,25],"acid":[4,26],"detection":[5,37],"is":[6,22,159,164],"increasing":[7],"worldwide":[8],"as":[9],"people":[10],"are":[11],"impacted":[12],"by":[13],"infectious":[14],"diseases.":[15],"Digital":[16],"polymerase":[17],"chain":[18],"reaction":[19,91,147],"(dPCR),":[20],"which":[21],"a":[23,32,65],"revolutionized":[24],"absolute":[27],"quantitative":[28],"analysis":[29],"technology,":[30],"plays":[31],"significant":[33],"role":[34],"in":[35,96,152],"the":[36,42,80,85,101,104,109,115,126,132,146,161],"of":[38,93,118,131,149],"genetic":[39],"material.":[40],"However,":[41],"traditional":[43],"threshold-based":[44],"methods":[45],"fail":[46],"to":[47,71,89,137],"obtain":[48],"high-accuracy":[49],"segmentation":[50,117,135,176],"results":[51,168],"and":[52,84,128],"real-time":[53,116],"performance":[54,177],"from":[55],"dPCR":[56,74,94,133,150,180],"fluorescence":[57,75,181],"images":[58,95,151,182],"with":[59,73,183],"uneven":[60,119,184],"illumination.":[61,185],"Therefore,":[62],"we":[63],"proposed":[64,172],"robust":[66],"two-stage":[67],"automatic":[68],"thresholding":[69],"method":[70,78,83,88,106,112,123,142],"deal":[72],"images.":[76],"This":[77,141],"adopts":[79],"background":[81],"subtraction":[82],"improved":[86,110],"Otsu":[87,111],"extract":[90],"chambers":[92,148],"two":[97],"stages.":[98],"Especially":[99],"second":[102],"stage,":[103],"peak-weaken":[105],"based":[107],"on":[108],"greatly":[113],"improves":[114,125],"illumination":[120],"regions.":[121],"Our":[122],"significantly":[124],"accuracy":[127],"processing":[129],"speed":[130],"image":[134],"compared":[136],"several":[138],"mainstream":[139],"methods.":[140],"could":[143],"effectively":[144],"segment":[145],"complex":[153],"environments.":[154],"average":[156],"precision":[157],"rate":[158],"99.71%,":[160],"execution":[162],"time":[163],"0.139":[165],"s.":[166],"Experimental":[167],"demonstrate":[169],"that":[170],"our":[171],"strategy":[173],"has":[174],"superior":[175],"high-throughput":[179]},"cited_by_api_url":"https://api.openalex.org/works?filter=cites:W4225159435","counts_by_year":[{"year":2024,"cited_by_count":1}],"updated_date":"2025-01-16T05:27:52.939132","created_date":"2022-05-01"}