{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T13:55:44Z","timestamp":1724421344769},"reference-count":29,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T00:00:00Z","timestamp":1537315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017M3A7B4039936"],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ01252602"],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004083","name":"Ministry of Science ICT and Future Planning","doi-asserted-by":"publisher","award":["2018R1C1B3001553"],"id":[{"id":"10.13039\/501100004083","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"Since the increment of the threat to public health caused by foodborne pathogens, researches have been widely studied on developing the miniaturized detection system for the on-site pathogen detection. In the study, we focused on the development of portable, robust, and disposable film-based polymerase chain reaction (PCR) chip containing a multiplex chamber for simultaneous gene amplification. In order to simply fabricate and operate a film-based PCR chip, different kinds of PCR chambers were designed and fabricated using polyethylene terephthalate (PET) and polyvinyl chloride (PVC) adhesive film, in comparison with commercial PCR, which employs a stereotyped system at a bench-top scale. No reagent leakage was confirmed during the PCR thermal cycling using the film PCR chip, which indicates that the film PCR chip is structurally stable for rapid heat cycling for DNA amplification. Owing to use of the thin film to fabricate the PCR chip, we are able to realize fast thermal transfer from the heat block that leads to short PCR amplification time. Moreover, using the film PCR chip, we could even amplify the target pathogen with 10 CFU mL\u22121. The artificially infected milk with various concentration of Bacillus cereus was successfully amplified on a single film PCR chip. On the basis of the reliable results, the developed film PCR chip could be a useful tool as a POCT device to detect foodborne pathogens via genetic analysis.<\/jats:p>","DOI":"10.3390\/s18093158","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T14:50:31Z","timestamp":1537368631000},"page":"3158","source":"Crossref","is-referenced-by-count":15,"title":["A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen"],"prefix":"10.3390","volume":"18","author":[{"given":"Nam Ho","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering, Hanbat National University, Daejeon 34158, Korea"},{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Sun Young","family":"Lim","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Younseong","family":"Song","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Soon Woo","family":"Jeong","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Seol Yi","family":"Shin","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Yong Tae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering & Biotechnology, Korea Polytechnic University, 237 Sangidaehak-ro, Siheung-si 15073, Gyeonggi-do, Korea"}]},{"given":"Tae Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Kyoung G.","family":"Lee","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Seok Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"given":"Yong-Jun","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, Hanbat National University, Daejeon 34158, Korea"}]},{"given":"Yoo Min","family":"Park","sequence":"additional","affiliation":[{"name":"Nano-Bio Application Team, National NanoFab Center (NNFC), 291 Deahak-ro, Yuseong-gu, Daejeon 34141, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vashist, S.K. 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