{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T20:02:27Z","timestamp":1743883347417},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,11,22]]},"abstract":"Abstract<\/jats:title>\n Motivation: Most non-coding RNAs are characterized by a specific secondary and tertiary structure that determines their function. Here, we investigate the folding energy of the secondary structure of non-coding RNA sequences, such as microRNA precursors, transfer RNAs and ribosomal RNAs in several eukaryotic taxa. Statistical biases are assessed by a randomization test, in which the predicted minimum free energy of folding is compared with values obtained for structures inferred from randomly shuffling the original sequences.<\/jats:p>\n Results: In contrast with transfer RNAs and ribosomal RNAs, the majority of the microRNA sequences clearly exhibit a folding free energy that is considerably lower than that for shuffled sequences, indicating a high tendency in the sequence towards a stable secondary structure. A possible usage of this statistical test in the framework of the detection of genuine miRNA sequences is discussed.<\/jats:p>\n Availability: The dataset, software and additional data files are freely available as supplementary information on our Website.<\/jats:p>\n Supplementary information: \u00a0http:\/\/www.psb.ugent.be\/bioinformatics\/<\/jats:p>","DOI":"10.1093\/bioinformatics\/bth374","type":"journal-article","created":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T00:25:10Z","timestamp":1088123110000},"page":"2911-2917","source":"Crossref","is-referenced-by-count":438,"title":["Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences"],"prefix":"10.1093","volume":"20","author":[{"given":"Eric","family":"Bonnet","sequence":"first","affiliation":[]},{"given":"Jan","family":"Wuyts","sequence":"additional","affiliation":[]},{"given":"Pierre","family":"Rouz\u00e9","sequence":"additional","affiliation":[]},{"given":"Yves","family":"Van de Peer","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2004,6,23]]},"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/17\/2911\/48906367\/bioinformatics_20_17_2911.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/17\/2911\/48906367\/bioinformatics_20_17_2911.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T15:58:24Z","timestamp":1674662304000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/20\/17\/2911\/186725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,23]]},"references-count":0,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2004,11,22]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/bth374","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2004,11,22]]},"published":{"date-parts":[[2004,6,23]]}}}