{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T08:40:26Z","timestamp":1696408826269},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T00:00:00Z","timestamp":1450656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Comput Chem"],"published-print":{"date-parts":[[2016,4,15]]},"abstract":"Dynamic characteristics of protein surfaces are among the factors determining their functional properties, including their potential participation in protein\u2010protein interactions. The presence of clusters of static residues\u2014\u201cstability patches\u201d (SPs)\u2014is a characteristic of protein surfaces involved in intermolecular recognition. The mechanism, by with SPs facilitate molecular recognition, however, remains unclear. Analyzing the surface dynamic properties of the growth hormone and of its high\u2010affinity variant we demonstrated that reshaping of the SPs landscape may be among the factors accountable for the improved affinity of this variant to the receptor. We hypothesized that SPs facilitate molecular recognition by moderating the conformational entropy of the unbound state, diminishing enthalpy\u2013entropy compensation upon binding, and by augmenting the favorable entropy of desolvation. SPs mapping emerges as a valuable tool for investigating the structural basis of the stability of protein complexes and for rationalizing experimental approaches, such as affinity maturation, aimed at improving it. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcc.24276","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T14:59:25Z","timestamp":1450709965000},"page":"913-919","source":"Crossref","is-referenced-by-count":3,"title":["The role of protein \u201cStability patches\u201d in molecular recognition: A case study of the human growth hormone\u2010receptor complex"],"prefix":"10.1002","volume":"37","author":[{"given":"Roman","family":"Osman","sequence":"first","affiliation":[{"name":"Department of Structural and Chemical Biology Icahn School of Medicine at Mount Sinai New York"}]},{"given":"Mihaly","family":"Mezei","sequence":"additional","affiliation":[{"name":"Department of Structural and Chemical Biology Icahn School of Medicine at Mount Sinai New York"}]},{"given":"Stanislav","family":"Engel","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences National Institute for Biotechnology in the Negev, Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}]}],"member":"311","published-online":{"date-parts":[[2015,12,21]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003028"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.11.009"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr800373w"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00501-3"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp0717185"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp209437j"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja307401r"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp409805p"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/B804734K"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(87)83303-9"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706407105"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752-0509-2-48"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1669"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1611"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.8.3407"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603826200"},{"key":"e_1_2_5_17_1","first-page":"231","volume-title":"Supramolecular Structure and Function 8","author":"Verkhivker G.","year":"2004"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1612"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5348"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00898-2"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1549776"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403336101"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2014.01.012"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1907025"},{"key":"e_1_2_5_25_1","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1002\/jcc.540050604","author":"Mezei M.","year":"1984","journal-title":"J. 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