Team:Austin UTexas/Contribution

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Contribution

We updated 304 existing biobrick pages with new documentation: burden values

The 2020 Austin_UTexas team has updated 304 existing pages on the registry with new information that was previously generated by the 2019 Austin_UTexas team's data. The 2019 Austin_UTexas team used a standardized method to measure the burden for hundreds of BioBricks in the registry. They transformed 304 iGEM BioBricks into E. coli host cells and measured the maximum growth rates of the resulting strains by monitoring OD600 in a microplate reader. They then performed a procedure to normalize measurements between different assays to produce the final burden values, which are reported as the mean percent reduction in growth rate ± 95% confidence intervals. The burden values calculated by the 2019 team were never documented on the BioBrick pages. So, this year, we updated all 304 pages with each Biobrick's burden value (percent reduction in growth rate), along with an interpretation of each value, under the Functional Parameters heading. A majority of the frequently used BioBricks, promoters, ribosome binding sites, terminators, and coding region parts now have burden values on their pages. All of the burden values, and their corresponding links to the registry pages, are also shown in the tables below.



This information is important to share because iGEM teams and synthetic biologists frequently use and depend on the BioBricks from the registry. Parts that impose significant burden on their host cells are likely to mutate to relieve this burden. This is concerning not only to bench scientists working with small lab cultures but also to scientists working with large bioreactor volumes of culture containing these BioBricks. The burden values are very informative, and future BioBrick users can now access and use the values to anticipate whether those parts are reliable enough for their project goals. They can also use the standardized "burden-o-meter" protocol to measure the burden of any other Biobricks that they are interested in.




The reported burden values were divided into three categories:

• No significant burden

• Significant burden: 0-20%

• Significant burden: >20%




Interpretations of Burden Values

Biobricks with no significant burden are ones that did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes.

BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they are used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable.




For More Information...

Refer to any one of the BBa_K3174002↗ - BBa_K3174007↗ pages for more information (under the Characterization heading) on the methods and other conclusions from the large-scale measurement project conducted by the 2019 Austin_UTexas team↗.




Burden Values

    

Biobricks with No Burden

Link to Biobrick Page	Biobrick Accession	Burden value (mean ± 95% CI)
http://parts.igem.org/Part:BBa_K519010	K519010	-0.1 ± 3.9%
http://parts.igem.org/Part:BBa_K1150038	K1150038	-0.1 ± 8.1%
http://parts.igem.org/Part:BBa_K576003	K576003	-0.2 ± 10.3%
http://parts.igem.org/Part:BBa_K648029	K648029	-0.2 ± 4.4%
http://parts.igem.org/Part:BBa_K538000	K538000	-0.2 ± 4%
http://parts.igem.org/Part:BBa_B0040	B0040	-0.2 ± 9.1%
http://parts.igem.org/Part:BBa_K538004	K538004	-0.3 ± 0.2%
http://parts.igem.org/Part:BBa_I712088	I712088	-0.3 ± 20.9%
http://parts.igem.org/Part:BBa_E0034	E0034	-0.3 ± 3.7%
http://parts.igem.org/Part:BBa_K738002	K738002	-0.4 ± 1.8%
http://parts.igem.org/Part:BBa_R0071	R0071	-0.4 ± 2.2%
http://parts.igem.org/Part:BBa_K784006	K784006	-0.4 ± 2.3%
http://parts.igem.org/Part:BBa_K747007	K747007	-0.4 ± 2.7%
http://parts.igem.org/Part:BBa_C0060	C0060	-0.4 ± 2.9%
http://parts.igem.org/Part:BBa_E0022	E0022	-0.4 ± 5.4%
http://parts.igem.org/Part:BBa_I732920	I732920	-0.5 ± 0.7%
http://parts.igem.org/Part:BBa_P10302	P10302	-0.5 ± 2.1%
http://parts.igem.org/Part:BBa_B0012	B0012	-0.5 ± 2.4%
http://parts.igem.org/Part:BBa_K539421	K539421	-0.5 ± 2.8%
http://parts.igem.org/Part:BBa_K747022	K747022	-0.6 ± 1.9%
http://parts.igem.org/Part:BBa_J23119	J23119	-0.6 ± 11%
http://parts.igem.org/Part:BBa_K398331	K398331	-0.6 ± 2.5%
http://parts.igem.org/Part:BBa_K592018	K592018	-0.7 ± 1.9%
http://parts.igem.org/Part:BBa_S03749	S03749	-0.8 ± 1.5%
http://parts.igem.org/Part:BBa_K936013	K936013	-0.8 ± 2.2%
http://parts.igem.org/Part:BBa_K823017	K823017	-0.8 ± 7%
http://parts.igem.org/Part:BBa_K747011	K747011	-0.9 ± 1.3%
http://parts.igem.org/Part:BBa_K567018	K567018	-0.9 ± 2.1%
http://parts.igem.org/Part:BBa_K624003	K624003	-0.9 ± 6.8%
http://parts.igem.org/Part:BBa_K654058	K654058	-0.9 ± 7.4%
http://parts.igem.org/Part:BBa_S04602	S04602	-1 ± 1.2%
http://parts.igem.org/Part:BBa_K523003	K523003	-1 ± 2.8%
http://parts.igem.org/Part:BBa_K1150039	K1150039	-1 ± 3.8%
http://parts.igem.org/Part:BBa_K747020	K747020	-1 ± 4.3%
http://parts.igem.org/Part:BBa_E1010	E1010	-1.1 ± 2.5%
http://parts.igem.org/Part:BBa_K808025	K808025	-1.1 ± 4.8%
http://parts.igem.org/Part:BBa_K817023	K817023	-1.2 ± 1.3%
http://parts.igem.org/Part:BBa_K567008	K567008	-1.2 ± 2.1%
http://parts.igem.org/Part:BBa_K1150043	K1150043	-1.2 ± 2.6%
http://parts.igem.org/Part:BBa_J61100	J61100	-1.2 ± 24.4%
http://parts.igem.org/Part:BBa_K812032	K812032	-1.2 ± 3.8%
http://parts.igem.org/Part:BBa_E0030	E0030	-1.2 ± 5%
http://parts.igem.org/Part:BBa_K624004	K624004	-1.3 ± 0.7%
http://parts.igem.org/Part:BBa_K575021	K575021	-1.3 ± 4.7%
http://parts.igem.org/Part:BBa_J45219	J45219	-1.3 ± 6.1%
http://parts.igem.org/Part:BBa_I759019	I759019	-1.4 ± 3.6%
http://parts.igem.org/Part:BBa_K627011	K627011	-1.4 ± 3.8%
http://parts.igem.org/Part:BBa_K733008	K733008	-1.5 ± 5%
http://parts.igem.org/Part:BBa_K817018	K817018	-1.7 ± 3.7%
http://parts.igem.org/Part:BBa_R0084	R0084	-1.7 ± 7.7%
http://parts.igem.org/Part:BBa_K541506	K541506	-1.8 ± 1.2%
http://parts.igem.org/Part:BBa_I759018	I759018	-1.8 ± 4.9%
http://parts.igem.org/Part:BBa_I751100	I751100	-1.9 ± 4.5%
http://parts.igem.org/Part:BBa_K569017	K569017	-2 ± 1.6%
http://parts.igem.org/Part:BBa_K817024	K817024	-2 ± 3%
http://parts.igem.org/Part:BBa_K598014	K598014	-2.1 ± 2.4%
http://parts.igem.org/Part:BBa_B0010	B0010	-2.1 ± 3.4%
http://parts.igem.org/Part:BBa_R0011	R0011	-2.2 ± 1.5%
http://parts.igem.org/Part:BBa_K525710	K525710	-2.2 ± 3.5%
http://parts.igem.org/Part:BBa_R0063	R0063	-2.3 ± 4.1%
http://parts.igem.org/Part:BBa_K1150040	K1150040	-2.3 ± 4.3%
http://parts.igem.org/Part:BBa_K777117	K777117	-2.4 ± 2.4%
http://parts.igem.org/Part:BBa_K731722	K731722	-2.7 ± 3.6%
http://parts.igem.org/Part:BBa_J45299	J45299	-2.7 ± 4.4%
http://parts.igem.org/Part:BBa_K909007	K909007	-2.9 ± 1.8%
http://parts.igem.org/Part:BBa_K607035	K607035	-2.9 ± 3.4%
http://parts.igem.org/Part:BBa_K516022	K516022	-3.1 ± 3.2%
http://parts.igem.org/Part:BBa_K914001	K914001	-3.1 ± 7.8%
http://parts.igem.org/Part:BBa_K660610	K660610	-3.5 ± 5.8%
http://parts.igem.org/Part:BBa_K314110	K314110	-3.5 ± 5.9%
http://parts.igem.org/Part:BBa_R0082	R0082	-5.4 ± 3.7%
http://parts.igem.org/Part:BBa_R0078	R0078	-5.4 ± 5.6%
http://parts.igem.org/Part:BBa_J23108	J23108	0.0 ± 1.5%
http://parts.igem.org/Part:BBa_K606017	K606017	0.0 ± 1.5%
http://parts.igem.org/Part:BBa_K733009	K733009	0.0 ± 2.6%
http://parts.igem.org/Part:BBa_R0040	R0040	0.0 ± 3%
http://parts.igem.org/Part:BBa_K784014	K784014	0.1 ± 2.4%
http://parts.igem.org/Part:BBa_K539119	K539119	0.1 ± 3.5%
http://parts.igem.org/Part:BBa_R0062	R0062	0.1 ± 6%
http://parts.igem.org/Part:BBa_B0030	B0030	0.2 ± 0.9%
http://parts.igem.org/Part:BBa_I6057	I6057	0.2 ± 2.1%
http://parts.igem.org/Part:BBa_K1033910	K1033910	0.2 ± 2.4%
http://parts.igem.org/Part:BBa_K516031	K516031	0.2 ± 4.2%
http://parts.igem.org/Part:BBa_K747018	K747018	0.2 ± 4.9%
http://parts.igem.org/Part:BBa_K515007	K515007	0.2 ± 5.3%
http://parts.igem.org/Part:BBa_K525998	K525998	0.2 ± 5.4%
http://parts.igem.org/Part:BBa_K540001	K540001	0.2 ± 5.4%
http://parts.igem.org/Part:BBa_K584000	K584000	0.2 ± 5.4%
http://parts.igem.org/Part:BBa_J52040	J52040	0.3 ± 2.2%
http://parts.igem.org/Part:BBa_I746375	I746375	0.3 ± 2.4%
http://parts.igem.org/Part:BBa_E0020	E0020	0.3 ± 2.8%
http://parts.igem.org/Part:BBa_K592011	K592011	0.3 ± 3.3%
http://parts.igem.org/Part:BBa_K1150041	K1150041	0.3 ± 4.5%
http://parts.igem.org/Part:BBa_J45319	J45319	0.4 ± 2.9%
http://parts.igem.org/Part:BBa_J23109	J23109	0.4 ± 3.6%
http://parts.igem.org/Part:BBa_R0080	R0080	0.5 ± 1.4%
http://parts.igem.org/Part:BBa_C0052	C0052	0.5 ± 2.6%
http://parts.igem.org/Part:BBa_K808003	K808003	0.5 ± 3.6%
http://parts.igem.org/Part:BBa_K936000	K936000	0.5 ± 3%
http://parts.igem.org/Part:BBa_K747004	K747004	0.5 ± 4.7%
http://parts.igem.org/Part:BBa_B0013	B0013	0.6 ± 0.8%
http://parts.igem.org/Part:BBa_C0012	C0012	0.6 ± 2.1%
http://parts.igem.org/Part:BBa_K592016	K592016	0.6 ± 3.8%
http://parts.igem.org/Part:BBa_K517002	K517002	0.6 ± 4.4%
http://parts.igem.org/Part:BBa_I13502	I13502	0.6 ± 6.2%
http://parts.igem.org/Part:BBa_K876000	K876000	0.7 ± 3.7%
http://parts.igem.org/Part:BBa_K747010	K747010	0.8 ± 12.3%
http://parts.igem.org/Part:BBa_K875009	K875009	0.8 ± 2.2%
http://parts.igem.org/Part:BBa_C0160	C0160	0.8 ± 5.7%
http://parts.igem.org/Part:BBa_I15017	I15017	0.8 ± 5.7%
http://parts.igem.org/Part:BBa_K1415006	K1415006	0.9 ± 1%
http://parts.igem.org/Part:BBa_K773004	K773004	0.9 ± 12.5%
http://parts.igem.org/Part:BBa_K1188002	K1188002	1 ± 1.4%
http://parts.igem.org/Part:BBa_K863206	K863206	1 ± 4.1%
http://parts.igem.org/Part:BBa_K747021	K747021	1 ± 9.9%
http://parts.igem.org/Part:BBa_K538101	K538101	1.1 ± 4.7%
http://parts.igem.org/Part:BBa_I13504	I13504	1.1 ± 5.4%
http://parts.igem.org/Part:BBa_R0065	R0065	1.2 ± 2.8%
http://parts.igem.org/Part:BBa_K525306	K525306	1.2 ± 3.1%
http://parts.igem.org/Part:BBa_K588001	K588001	1.2 ± 5.6%
http://parts.igem.org/Part:BBa_R0079	R0079	1.2 ± 7.7%
http://parts.igem.org/Part:BBa_B0031	B0031	1.3 ± 1.1%
http://parts.igem.org/Part:BBa_K648013	K648013	1.3 ± 1.4%
http://parts.igem.org/Part:BBa_K145302	K145302	1.3 ± 2.1%
http://parts.igem.org/Part:BBa_K516030	K516030	1.3 ± 2.5%
http://parts.igem.org/Part:BBa_K747002	K747002	1.3 ± 2.6%
http://parts.igem.org/Part:BBa_S03154	S03154	1.4 ± 10.3%
http://parts.igem.org/Part:BBa_C0061	C0061	1.4 ± 12.9%
http://parts.igem.org/Part:BBa_I746381	I746381	1.4 ± 4.3%
http://parts.igem.org/Part:BBa_K624000	K624000	1.4 ± 5.9%
http://parts.igem.org/Part:BBa_K592009	K592009	1.4 ± 6.5%
http://parts.igem.org/Part:BBa_S05060	S05060	1.4 ± 6%
http://parts.igem.org/Part:BBa_B0034	B0034	1.5 ± 13.1%
http://parts.igem.org/Part:BBa_K145001	K145001	1.5 ± 3.6%
http://parts.igem.org/Part:BBa_K590034	K590034	1.5 ± 5.8%
http://parts.igem.org/Part:BBa_K747019	K747019	1.5 ± 7.8%
http://parts.igem.org/Part:BBa_K567015	K567015	1.6 ± 2.2%
http://parts.igem.org/Part:BBa_K763001	K763001	1.6 ± 6.9%
http://parts.igem.org/Part:BBa_K808032	K808032	1.6 ± 6.9%
http://parts.igem.org/Part:BBa_K575020	K575020	1.7 ± 4.2%
http://parts.igem.org/Part:BBa_K523004	K523004	1.7 ± 6.7%
http://parts.igem.org/Part:BBa_E0024	E0024	1.8 ± 12.4%
http://parts.igem.org/Part:BBa_K611026	K611026	1.8 ± 4.6%
http://parts.igem.org/Part:BBa_K577894	K577894	1.8 ± 5.2%
http://parts.igem.org/Part:BBa_K1033916	K1033916	1.9 ± 2.5%
http://parts.igem.org/Part:BBa_K617003	K617003	1.9 ± 3.2%
http://parts.igem.org/Part:BBa_K747012	K747012	1.9 ± 3.5%
http://parts.igem.org/Part:BBa_R0010	R0010	1.9 ± 39.8%
http://parts.igem.org/Part:BBa_K316003	K316003	13.8 ± 13.4%
http://parts.igem.org/Part:BBa_I759005	I759005	17.8 ± 16%
http://parts.igem.org/Part:BBa_K523002	K523002	2 ± 3.8%
http://parts.igem.org/Part:BBa_K634001	K634001	2 ± 4.1%
http://parts.igem.org/Part:BBa_K873002	K873002	2 ± 7.8%
http://parts.igem.org/Part:BBa_K914003	K914003	2 ± 7%
http://parts.igem.org/Part:BBa_K137019	K137019	2.1 ± 1.7%
http://parts.igem.org/Part:BBa_K914011	K914011	2.1 ± 1.9%
http://parts.igem.org/Part:BBa_K608007	K608007	2.1 ± 2.5%
http://parts.igem.org/Part:BBa_K325903	K325903	2.1 ± 6.2%
http://parts.igem.org/Part:BBa_C0261	C0261	2.2 ± 1.6%
http://parts.igem.org/Part:BBa_J52011	J52011	2.2 ± 2.3%
http://parts.igem.org/Part:BBa_J45199	J45199	2.2 ± 2.7%
http://parts.igem.org/Part:BBa_K819010	K819010	2.2 ± 3.4%
http://parts.igem.org/Part:BBa_K808000	K808000	2.2 ± 4.3%
http://parts.igem.org/Part:BBa_K1218003	k1218003	2.2 ± 5.1%
http://parts.igem.org/Part:BBa_K731520	K731520	2.3 ± 3.4%
http://parts.igem.org/Part:BBa_K525406	K525406	2.4 ± 4%
http://parts.igem.org/Part:BBa_E0032	E0032	2.4 ± 6.1%
http://parts.igem.org/Part:BBa_K1150001	K1150001	2.4 ± 6.4%
http://parts.igem.org/Part:BBa_K1061003	K1061003	2.5 ± 11.9%
http://parts.igem.org/Part:BBa_K541505	K541505	2.5 ± 7.5%
http://parts.igem.org/Part:BBa_K747023	K747023	2.6 ± 5.9%
http://parts.igem.org/Part:BBa_K575015	K575015	2.6 ± 6.4%
http://parts.igem.org/Part:BBa_K1184000	K1184000	2.7 ± 3.2%
http://parts.igem.org/Part:BBa_K819017	K819017	2.7 ± 3.2%
http://parts.igem.org/Part:BBa_K608006	K608006	2.7 ± 3.9%
http://parts.igem.org/Part:BBa_E0422	E0422	2.8 ± 11.6%
http://parts.igem.org/Part:BBa_K575008	K575008	2.8 ± 2.5%
http://parts.igem.org/Part:BBa_K775000	K775000	2.8 ± 6.2%
http://parts.igem.org/Part:BBa_K895004	K895004	2.8 ± 8%
http://parts.igem.org/Part:BBa_K861090	K861090	2.8 ± 9%
http://parts.igem.org/Part:BBa_K747005	K747005	2.9 ± 3.3%
http://parts.igem.org/Part:BBa_K608351	K608351	2.9 ± 3.8%
http://parts.igem.org/Part:BBa_B0011	B0011	22.1 ± 24.3%
http://parts.igem.org/Part:BBa_I742111	I742111	22.7 ± 13.7%
http://parts.igem.org/Part:BBa_I13600	I13600	3 ± 10.7%
http://parts.igem.org/Part:BBa_C0040	C0040	3 ± 4.8%
http://parts.igem.org/Part:BBa_B0032	B0032	3 ± 6.2%
http://parts.igem.org/Part:BBa_K861173	K861173	3.1 ± 2.6%
http://parts.igem.org/Part:BBa_K538104	K538104	3.2 ± 10.8%
http://parts.igem.org/Part:BBa_K525404	K525404	3.2 ± 5.3%
http://parts.igem.org/Part:BBa_K517005	K517005	3.3 ± 4.2%
http://parts.igem.org/Part:BBa_J52022	J52022	3.4 ± 4.2%
http://parts.igem.org/Part:BBa_K1806004	K1806004	3.5 ± 4.5%
http://parts.igem.org/Part:BBa_I13001	I13001	3.5 ± 5.7%
http://parts.igem.org/Part:BBa_J61101	J61101	3.5 ± 8.8%
http://parts.igem.org/Part:BBa_E0036	E0036	3.6 ± 2.3%
http://parts.igem.org/Part:BBa_R1062	R1062	3.6 ± 3.1%
http://parts.igem.org/Part:BBa_K581005	K581005	3.8 ± 3.9%
http://parts.igem.org/Part:BBa_K115001	K115001	3.8 ± 7.2%
http://parts.igem.org/Part:BBa_I746200	I746200	3.9 ± 2.9%
http://parts.igem.org/Part:BBa_K517003	K517003	3.9 ± 4.2%
http://parts.igem.org/Part:BBa_K1061001	K1061001	4.1 ± 8.4%
http://parts.igem.org/Part:BBa_B1006	B1006	4.3 ± 4.4%
http://parts.igem.org/Part:BBa_K118000	K118000	4.3 ± 4.6%
http://parts.igem.org/Part:BBa_B0025	B0025	4.3 ± 6.9%
http://parts.igem.org/Part:BBa_B0033	B0033	4.6 ± 4.2%
http://parts.igem.org/Part:BBa_K895000	K895000	4.7 ± 5.4%
http://parts.igem.org/Part:BBa_I759020	I759020	4.7 ± 7.1%
http://parts.igem.org/Part:BBa_R1051	R1051	4.8 ± 6.9%
http://parts.igem.org/Part:BBa_K747003	K747003	4.9 ± 7.8%
http://parts.igem.org/Part:BBa_K784013	K784013	5 ± 10.6%
http://parts.igem.org/Part:BBa_B0015	B0015	5 ± 19.2%
http://parts.igem.org/Part:BBa_K747001	K747001	5 ± 4.3%
http://parts.igem.org/Part:BBa_K523015	K523015	5.1 ± 4.7%
http://parts.igem.org/Part:BBa_K541503	K541503	5.2 ± 5.4%
http://parts.igem.org/Part:BBa_E0420	E0420	5.2 ± 6.2%
http://parts.igem.org/Part:BBa_K844007	K844007	5.2 ± 7.3%
http://parts.igem.org/Part:BBa_K747009	K747009	5.4 ± 6.3%
http://parts.igem.org/Part:BBa_K523000	K523000	5.7 ± 6.1%
http://parts.igem.org/Part:BBa_K611025	K611025	5.7 ± 6.1%
http://parts.igem.org/Part:BBa_K773003	K773003	5.7 ± 9.3%
http://parts.igem.org/Part:BBa_R0083	R0083	5.8 ± 7%
http://parts.igem.org/Part:BBa_C0050	C0050	5.9 ± 5.1%
http://parts.igem.org/Part:BBa_I13601	I13601	5.9 ± 9.3%
http://parts.igem.org/Part:BBa_I13500	I13500	6.1 ± 5.6%
http://parts.igem.org/Part:BBa_K1166002	K1166002	6.1 ± 6.4%
http://parts.igem.org/Part:BBa_K733001	K733001	6.3 ± 10.6%
http://parts.igem.org/Part:BBa_I0500	I0500	6.7 ± 9.5%
http://parts.igem.org/Part:BBa_K844006	K844006	7 ± 10.2%
http://parts.igem.org/Part:BBa_K568004	K568004	7 ± 5.8%
http://parts.igem.org/Part:BBa_B0023	B0023	7.4 ± 6.5%
http://parts.igem.org/Part:BBa_K844008	K844008	7.6 ± 11.5%
http://parts.igem.org/Part:BBa_K575014	K575014	7.9 ± 16.1%
http://parts.igem.org/Part:BBa_K118019	K118019	8 ± 8.3%
http://parts.igem.org/Part:BBa_K797001	K797001	8.3 ± 19%
http://parts.igem.org/Part:BBa_K115002	K115002	8.4 ± 17.7%
http://parts.igem.org/Part:BBa_K748002	K748002	9.3 ± 9.1%
http://parts.igem.org/Part:BBa_K523006	K523006	9.6 ± 17.9%








    

Biobricks with Significant Burden (0-20%)

Link to Biobrick Page	Biobrick Accession	Burden value (mean ± 95% CI)
http://parts.igem.org/Part:BBa_E0026	E0026	3.1 ± 1.3%
http://parts.igem.org/Part:BBa_I712058	I712058	3.4 ± 0.5%
http://parts.igem.org/Part:BBa_K773002	K773002	4.2 ± 3.0%
http://parts.igem.org/Part:BBa_K747008	K747008	4.3 ± 2.0%
http://parts.igem.org/Part:BBa_I759016	I759016	5.0 ± 2.5%
http://parts.igem.org/Part:BBa_K774001	K774001	5.0 ± 2.5%
http://parts.igem.org/Part:BBa_K608004	K608004	5.3 ± 2.2%
http://parts.igem.org/Part:BBa_K398326	K398326	5.3 ± 2.4%
http://parts.igem.org/Part:BBa_K608003	K608003	5.6 ± 1.5%
http://parts.igem.org/Part:BBa_K747000	K747000	5.6 ± 3.8%
http://parts.igem.org/Part:BBa_K553003	K553003	5.7 ± 3.1%
http://parts.igem.org/Part:BBa_I746391	I746391	5.9 ± 4.4%
http://parts.igem.org/Part:BBa_K118028	K118028	6.9 ± 3.2%
http://parts.igem.org/Part:BBa_K516011	K516011	7.0 ± 3.1%
http://parts.igem.org/Part:BBa_I13002	I13002	7.5 ± 3.2%
http://parts.igem.org/Part:BBa_K581008	K581008	7.7 ± 4.9%
http://parts.igem.org/Part:BBa_K1150042	K1150042	7.8 ± 2.1%
http://parts.igem.org/Part:BBa_K575009	K575009	8.0 ± 4.3%
http://parts.igem.org/Part:BBa_K325909	K325909	8.3 ± 2.2%
http://parts.igem.org/Part:BBa_I13602	I13602	8.4 ± 3.2%
http://parts.igem.org/Part:BBa_K608002	K608002	8.5 ± 5.1%
http://parts.igem.org/Part:BBa_I746908	I746908	8.6 ± 2.4%
http://parts.igem.org/Part:BBa_K516132	K516132	8.7 ± 4.8%
http://parts.igem.org/Part:BBa_E0430	E0430	9.0 ± 4.5%
http://parts.igem.org/Part:BBa_K137076	K137076	9.5 ± 4.4%
http://parts.igem.org/Part:BBa_K523023	K523023	9.8 ± 3.9%
http://parts.igem.org/Part:BBa_K118024	K118024	9.8 ± 5.8%
http://parts.igem.org/Part:BBa_I712669	I712669	10.1 ± 4.5%
http://parts.igem.org/Part:BBa_K325218	K325218	10.8 ± 7.3%
http://parts.igem.org/Part:BBa_K617004	K617004	11.6 ± 1.5%
http://parts.igem.org/Part:BBa_K861172	K861172	13.4 ± 2.5%
http://parts.igem.org/Part:BBa_K608011	K608011	13.7 ± 5.4%
http://parts.igem.org/Part:BBa_K541501	K541501	14.4 ± 3.6%
http://parts.igem.org/Part:BBa_K639003	K639003	14.8 ± 2.8%
http://parts.igem.org/Part:BBa_K731721	K731721	14.8 ± 4.4%
http://parts.igem.org/Part:BBa_K1149051	K1149051	15.0 ± 8.4%
http://parts.igem.org/Part:BBa_I0466	I0466	16.4 ± 10.1%
http://parts.igem.org/Part:BBa_K137018	K137018	16.8 ± 8.2%
http://parts.igem.org/Part:BBa_C0051	C0051	17.0 ± 8.2%
http://parts.igem.org/Part:BBa_I761014	I761014	17.5 ± 5%
http://parts.igem.org/Part:BBa_K523013	K523013	18.3 ± 8.8%
http://parts.igem.org/Part:BBa_K733013	K733013	19.5 ± 3.3%








    

Biobricks with Significant Burden (>20%)

Link to Biobrick Page	Biobrick Accession	Burden value (mean ± 95% CI)
http://parts.igem.org/Part:BBa_K395602	K395602	20.3 ± 1.9%
http://parts.igem.org/Part:BBa_K541502	K541502	24.6 ± 3.2%
http://parts.igem.org/Part:BBa_I759014	I759014	26.8 ± 5.8%
http://parts.igem.org/Part:BBa_K608012	K608012	27.1 ± 4.7%
http://parts.igem.org/Part:BBa_C0053	C0053	27.1 ± 6.5%
http://parts.igem.org/Part:BBa_K880005	K880005	27.5 ± 8.6%
http://parts.igem.org/Part:BBa_C0056	C0056	28.2 ± 3.9%
http://parts.igem.org/Part:BBa_K346000	K346000	29.1 ± 10.4%
http://parts.igem.org/Part:BBa_I759017	I759017	29.5 ± 8.3%
http://parts.igem.org/Part:BBa_J36335	J36335	30.2 ± 12.2%
http://parts.igem.org/Part:BBa_K592020	K592020	31.8 ± 5.0%
http://parts.igem.org/Part:BBa_K541526	K541526	32.9 ± 7.8%
http://parts.igem.org/Part:BBa_J61000	J61000	33.4 ± 4.1%
http://parts.igem.org/Part:BBa_K515100	K515100	33.9 ± 15.9%
http://parts.igem.org/Part:BBa_K608010	K608010	34.1 ± 7.7%
http://parts.igem.org/Part:BBa_K523020	K523020	38.3 ± 8.7%
http://parts.igem.org/Part:BBa_K523014	K523014	39.6 ± 4.7%
http://parts.igem.org/Part:BBa_K733010	K733010	46.0 ± 6.2%
http://parts.igem.org/Part:BBa_K523022	K523022	51.7 ± 19.2%