Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
- PMID: 16896339
- PMCID: PMC2700296
- DOI: 10.1038/nmeth929
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
Abstract
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
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Comment in
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Single-molecule mountains yield nanoscale cell images.Nat Methods. 2006 Oct;3(10):781-2. doi: 10.1038/nmeth1006-781. Nat Methods. 2006. PMID: 16990808 Free PMC article.
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The limits of light.Nat Rev Mol Cell Biol. 2010 Oct;11(10):678. doi: 10.1038/nrm2989. Nat Rev Mol Cell Biol. 2010. PMID: 20861874 No abstract available.
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