Mechanical properties of cultured human airway smooth muscle cells from 0.05 to 0.4 Hz
- PMID: 11007604
- DOI: 10.1152/jappl.2000.89.4.1619
Mechanical properties of cultured human airway smooth muscle cells from 0.05 to 0.4 Hz
Abstract
We investigated the rheological properties of living human airway smooth muscle cells in culture and monitored the changes in rheological properties induced by exogenous stimuli. We oscillated small magnetic microbeads bound specifically to integrin receptors and computed the storage modulus (G') and loss modulus (G") from the applied torque and the resulting rotational motion of the beads as determined from their remanent magnetic field. Under baseline conditions, G' increased weakly with frequency, whereas G" was independent of the frequency. The cell was predominantly elastic, with the ratio of G" to G' (defined as eta) being approximately 0. 35 at all frequencies. G' and G" increased together after contractile activation and decreased together after deactivation, whereas eta remained unaltered in each case. Thus elastic and dissipative stresses were coupled during changes in contractile activation. G' and G" decreased with disruption of the actin fibers by cytochalasin D, but eta increased. These results imply that the mechanisms for frictional energy loss and elastic energy storage in the living cell are coupled and reside within the cytoskeleton.
Comment in
-
Biology lessons from oscillatory cell mechanics.J Appl Physiol (1985). 2000 Oct;89(4):1617-8. doi: 10.1152/jappl.2000.89.4.1617. J Appl Physiol (1985). 2000. PMID: 11007603 No abstract available.
Similar articles
-
Effect of the cytoskeletal prestress on the mechanical impedance of cultured airway smooth muscle cells.J Appl Physiol (1985). 2002 Apr;92(4):1443-50. doi: 10.1152/japplphysiol.00782.2001. J Appl Physiol (1985). 2002. PMID: 11896008
-
Probing the viscoelastic behavior of cultured airway smooth muscle cells with atomic force microscopy: stiffening induced by contractile agonist.Biophys J. 2005 Apr;88(4):2994-3007. doi: 10.1529/biophysj.104.046649. Epub 2005 Jan 21. Biophys J. 2005. PMID: 15665124 Free PMC article.
-
Selected contribution: time course and heterogeneity of contractile responses in cultured human airway smooth muscle cells.J Appl Physiol (1985). 2001 Aug;91(2):986-94. doi: 10.1152/jappl.2001.91.2.986. J Appl Physiol (1985). 2001. PMID: 11457818 Review.
-
Viscoelastic and dynamic nonlinear properties of airway smooth muscle tissue: roles of mechanical force and the cytoskeleton.Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1227-37. doi: 10.1152/ajplung.00299.2005. Epub 2006 Jan 13. Am J Physiol Lung Cell Mol Physiol. 2006. PMID: 16414980
-
Ultrastructural basis of airway smooth muscle contraction.Can J Physiol Pharmacol. 2007 Jul;85(7):659-65. doi: 10.1139/Y07-052. Can J Physiol Pharmacol. 2007. PMID: 17823629 Review.
Cited by
-
Probing mechanical properties of Jurkat cells under the effect of ART using oscillating optical tweezers.PLoS One. 2015 Apr 30;10(4):e0126548. doi: 10.1371/journal.pone.0126548. eCollection 2015. PLoS One. 2015. PMID: 25928073 Free PMC article.
-
Nonlinear Elastic and Inelastic Properties of Cells.J Biomech Eng. 2020 Oct 1;142(10):100806. doi: 10.1115/1.4046863. J Biomech Eng. 2020. PMID: 32253428 Free PMC article. Review.
-
Particle uptake in cancer cells can predict malignancy and drug resistance using machine learning.Sci Adv. 2024 May 31;10(22):eadj4370. doi: 10.1126/sciadv.adj4370. Epub 2024 May 29. Sci Adv. 2024. PMID: 38809990 Free PMC article.
-
Optomechanical measurement of the stiffness of single adherent cells.Lab Chip. 2015 Sep 7;15(17):3460-4. doi: 10.1039/c5lc00444f. Epub 2015 Jul 29. Lab Chip. 2015. PMID: 26220705 Free PMC article.
-
Mechanical behavior in living cells consistent with the tensegrity model.Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7765-70. doi: 10.1073/pnas.141199598. Proc Natl Acad Sci U S A. 2001. PMID: 11438729 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
