A peptide microarray fabricated on a non-fouling phosphatidylcholine-polymer-coated surface for a high-fidelity analysis of a cellular kinome

Title:A peptide microarray fabricated on a non-fouling phosphatidylcholine-polymer-coated surface for a high-fidelity analysis of a cellular kinome

Volume: 20 Issue: 35

Author(s): H. Ikeda, J. Kamimoto, T. Yamamoto, A. Hata, Y. Otsubo, T. Niidome, M. Fukushima, T. Mori and Y. Katayama

Affiliation:

Keywords: Kinome peptide, microarray, non-fouling, phosphatidylcholine, protein kinase.

Abstract: Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.

Cite this article as:

Ikeda H., Kamimoto J., Yamamoto T., Hata A., Otsubo Y., Niidome T., Fukushima M., Mori T. and Katayama Y., A peptide microarray fabricated on a non-fouling phosphatidylcholine-polymer-coated surface for a high-fidelity analysis of a cellular kinome, Current Medicinal Chemistry 2013; 20 (35) . https://dx.doi.org/10.2174/09298673113209990142