Part:BBa_K773002

Proteorhodopsin

This part carries the gene for a proteorhodopsin pump, which can generate a proton motive force for making ATP.

Usage and Biology

This part was improved by Silvia Galvan of the iGEM UniTN Trento 2015 team. This part was previously found by other users to not express protein nor produce ATP. We improved this part by adding a strong RBS and using a different inducible promoter (arabinose, araC-pBAD). We were able to express the protein and functionally characterize it. The part now works! Check the experience page of this part to see our data.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 515
  Illegal XhoI site found at 88
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 277
  Illegal AgeI site found at 652
  
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 4.2 ± 3.0%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.