1.ȷԣͬʱӿ̣ԼʱͽǮ
šϢḻĴڣģⳣ¡PCR
ʾͼؿͬһ
SnapGeneͨDNAữϸڣʶܳʧ
2.ƾױȵĿԺͲٿԣʹǿ¡ҲܿӦԸ
÷ǷDNAеĿӽ
зḻע͵ͼɨǧעԵĴDNA
ͨ߶ȿӻIJģṹıȶԣȷϿ¡
3.ģԶ鵵ʡʱ䣬÷ͱ湤ɡЧҲЧ
Զɷḻͼʷ¼ɲ鿴б༭Ϳ¡
ͨʷϸڵṹǿЭ֪ʶ
ȫġܣÿһ
4.Խӿ¡Ļ֪ʶ
ֱ۵ļʶ¡еȱݣӶ
ʹԼģPCRSnapGeneԶ
רҵĿ¡ȷҪӿ¡Ŀȷ
5.Զ鿴
ȿSnapGeneеݿҲԶע
ͼϻͼϸʾøλ㡢ORFsϢ
עͺͿӻؼԶͼ
6.ȶԹߣ
ǿġȶԲο(Align to Reference)ߣ֤лṹģṹǷƥ
ʹÿ㷨нгɶ/رȶԣClustal OmegaMAFFTMUSCLET-Coffee
ͨCAP3SangerصȺ(contigs)
7.ģ֬
ƾзḻģ㷨SnapGeneȷӻ㽫ʵп龰
Ԫأ(Ӿ)Ӿ֬%ʱԼȫMW
¼ʶȤbandṩÿ(Ӿ)ӾϸƬϢ
8.Զͼλʷ¼
ֱ۵ؼ¼ʵ̺ĵ
ʷ¼ͼסͼʾʷ¼ɫ
Ƕĵеȫɻָʷ
9.ת
洢֯Сļͼ
洢ڿɹƷемЭͬ
ɶȡ͵ೣļʽСעͺԪ